8VDN
DNA Ligase 1 with nick dG:C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.977, 115.631, 126.175 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.520 - 2.390 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.24140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.554 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.440 |
High resolution limit [Å] | 2.390 | 2.390 |
Rpim | 0.031 | 0.510 |
Number of reflections | 38037 | 1880 |
<I/σ(I)> | 17.1 | 1.1 |
Completeness [%] | 99.4 | 100 |
Redundancy | 6.5 | |
CC(1/2) | 0.994 | 0.673 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 100 mM MES, pH 6.4, 100 mM Lithium acetate, 12% w/v PEG 3350 |