8VD3
Crystal Structure of D14 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2019-12-12 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.692, 69.267, 90.800 |
| Unit cell angles | 90.00, 93.38, 90.00 |
Refinement procedure
| Resolution | 45.320 - 2.000 |
| R-factor | 0.16495 |
| Rwork | 0.163 |
| R-free | 0.20724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.320 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36532 | 2459 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.15M Ammonium acetate, 0.01M Calcium chloride dihydrate, 0.1M Tris pH 8.5, 28% broad PEG smear from BCS (Molecular Dimensions) |
