8VBQ
Structure of bovine anti-HIV Fab Bess7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-20 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97741 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.002, 69.784, 87.521 |
Unit cell angles | 90.00, 103.52, 90.00 |
Refinement procedure
Resolution | 48.290 - 2.100 |
R-factor | 0.2333 |
Rwork | 0.232 |
R-free | 0.26770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.290 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.152 | 1.070 |
Rmeas | 0.179 | 1.280 |
Rpim | 0.093 | 0.703 |
Number of reflections | 28113 | 2607 |
<I/σ(I)> | 8.7 | 1.2 |
Completeness [%] | 98.8 | 92.4 |
Redundancy | 3.5 | 3 |
CC(1/2) | 0.992 | 0.376 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.095M sodium citrate, pH 5.6, 19% 2-propanol, 5% glycerol, 19% Peg4000 |