8VBQ
Structure of bovine anti-HIV Fab Bess7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.002, 69.784, 87.521 |
| Unit cell angles | 90.00, 103.52, 90.00 |
Refinement procedure
| Resolution | 48.290 - 2.100 |
| R-factor | 0.2333 |
| Rwork | 0.232 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.290 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.152 | 1.070 |
| Rmeas | 0.179 | 1.280 |
| Rpim | 0.093 | 0.703 |
| Number of reflections | 28113 | 2607 |
| <I/σ(I)> | 8.7 | 1.2 |
| Completeness [%] | 98.8 | 92.4 |
| Redundancy | 3.5 | 3 |
| CC(1/2) | 0.992 | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.095M sodium citrate, pH 5.6, 19% 2-propanol, 5% glycerol, 19% Peg4000 |
