8VBP
Structure of bovine anti-HIV Fab Bess4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.607, 70.283, 111.459 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.940 - 2.800 |
R-factor | 0.1984 |
Rwork | 0.197 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.576 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.940 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.191 | 0.805 |
Rmeas | 0.224 | 0.959 |
Rpim | 0.115 | 0.510 |
Number of reflections | 12710 | 1143 |
<I/σ(I)> | 7 | 3 |
Completeness [%] | 93.8 | 87.2 |
Redundancy | 3.5 | 3.3 |
CC(1/2) | 0.973 | 0.498 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 70% MPD, 0.1M Hepes, pH 7.5 |