8VBK
Structure of bovine anti-HIV Fab ElsE5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.261, 70.018, 109.082 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.860 - 1.890 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.860 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.118 | 1.090 |
| Rmeas | 0.123 | 1.140 |
| Rpim | 0.035 | 0.323 |
| Number of reflections | 34653 | 3286 |
| <I/σ(I)> | 13.8 | 2.4 |
| Completeness [%] | 98.7 | 0.949 |
| Redundancy | 12.2 | 12.1 |
| CC(1/2) | 0.998 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M sodium cacodylate, pH 5.5, 40% MPD, 5% Peg8000 |
