8VBK
Structure of bovine anti-HIV Fab ElsE5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.261, 70.018, 109.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.860 - 1.890 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.633 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.860 | 1.960 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.118 | 1.090 |
Rmeas | 0.123 | 1.140 |
Rpim | 0.035 | 0.323 |
Number of reflections | 34653 | 3286 |
<I/σ(I)> | 13.8 | 2.4 |
Completeness [%] | 98.7 | 0.949 |
Redundancy | 12.2 | 12.1 |
CC(1/2) | 0.998 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M sodium cacodylate, pH 5.5, 40% MPD, 5% Peg8000 |