8V9O

Imaging scaffold engineered to bind the therapeutic protein target BARD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979340
Spacegroup name	I 2 2 2
Unit cell lengths	128.010, 195.570, 228.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 3.810
R-factor	0.2002
Rwork	0.197
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.990	19.990	3.910
High resolution limit [Å]	3.810	17.020	3.810
Rmerge	0.129	0.029	2.051
Rmeas	0.139	0.032	2.237
Number of reflections	28155	137	1980
<I/σ(I)>	11.07
Completeness [%]	98.9
Redundancy	6.8
CC(1/2)	0.999	0.999	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.14M Calcium chloride , 0.07M Sodium Acetate 4.6 , 14% v/v Isopropanol , 30% v/v glycerol