8V96
GII.14 M7 norovirus protruding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.818, 91.835, 67.106 |
| Unit cell angles | 90.00, 92.98, 90.00 |
Refinement procedure
| Resolution | 42.580 - 1.440 |
| R-factor | 0.2081 |
| Rwork | 0.207 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.119 | 1.939 |
| Rmeas | 0.141 | 2.302 |
| Rpim | 0.076 | 1.228 |
| Total number of observations | 381270 | 17145 |
| Number of reflections | 113736 | 5241 |
| <I/σ(I)> | 6.5 | 0.6 |
| Completeness [%] | 98.4 | |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.993 | 0.163 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M BICINE 10% v/v MPD |
