8V96

GII.14 M7 norovirus protruding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-06-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95373
Spacegroup name	C 1 2 1
Unit cell lengths	105.818, 91.835, 67.106
Unit cell angles	90.00, 92.98, 90.00

Refinement procedure

Resolution	42.580 - 1.440
R-factor	0.2081
Rwork	0.207
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	1.460
High resolution limit [Å]	1.440	1.440
Rmerge	0.119	1.939
Rmeas	0.141	2.302
Rpim	0.076	1.228
Total number of observations	381270	17145
Number of reflections	113736	5241
<I/σ(I)>	6.5	0.6
Completeness [%]	98.4
Redundancy	3.4	3.3
CC(1/2)	0.993	0.163

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M BICINE 10% v/v MPD