8V7K

Human DNA polymerase eta-DNA-araC-ended primer ternary complex:reaction with 10 mM Mn2+ for 1800s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1272
Spacegroup name	P 61
Unit cell lengths	98.520, 98.520, 81.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.810 - 1.650
R-factor	0.2069
Rwork	0.206
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.321
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.810	37.810	1.750
High resolution limit [Å]	1.650	4.940	1.650
Rmerge	0.065	0.035	0.869
Rmeas	0.068	0.037	0.916
Number of reflections	53654	3958	17039
<I/σ(I)>	19.33
Completeness [%]	99.9
Redundancy	20.3
CC(1/2)	0.999	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES