8V7J

Human DNA polymerase eta-DNA-araC-ended primer ternary complex:reaction with 20 mM Mg2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-10-07
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.722, 98.722, 82.093
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.920 - 1.660
R-factor	0.2019
Rwork	0.201
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.920	37.920	1.770
High resolution limit [Å]	1.660	4.960	1.660
Rmerge	0.060	0.029	0.727
Rmeas	0.066	0.032	0.800
Number of reflections	53145	3944	16736
<I/σ(I)>	18.8
Completeness [%]	99.7
Redundancy	11.3
CC(1/2)	0.999	0.999	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES