8V7D

Human DNA polymerase eta-DNA-dT primer rCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-04-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	99.058, 99.058, 81.386
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.310 - 1.950
R-factor	0.1846
Rwork	0.182
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.310	42.310	1.860
High resolution limit [Å]	1.750	5.220	1.750
Rmerge	0.075	0.022	2.324
Rmeas	0.083	0.025	2.848
Number of reflections	33629	3393	14433
<I/σ(I)>	12.92
Completeness [%]	99.8
Redundancy	11.3
CC(1/2)	0.999	1.000	0.144

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES