8V7B

Human DNA polymerase eta-DNA-dT primer araCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-04-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.848, 98.848, 82.048
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.800 - 1.900
R-factor	0.1784
Rwork	0.176
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.056
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.800	42.800	1.800
High resolution limit [Å]	1.690	5.050	1.690
Rmerge	0.096	0.021	2.935
Rmeas	0.105	0.023	3.231
Number of reflections	36504	3760	16004
<I/σ(I)>	11.88
Completeness [%]	99.9
Redundancy	11.4
CC(1/2)	0.999	1.000	0.179

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES