8V7B
Human DNA polymerase eta-DNA-dT primer araCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-04-15 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 98.848, 98.848, 82.048 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.800 - 1.900 |
R-factor | 0.1784 |
Rwork | 0.176 |
R-free | 0.21460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.056 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.800 | 42.800 | 1.800 |
High resolution limit [Å] | 1.690 | 5.050 | 1.690 |
Rmerge | 0.096 | 0.021 | 2.935 |
Rmeas | 0.105 | 0.023 | 3.231 |
Number of reflections | 36504 | 3760 | 16004 |
<I/σ(I)> | 11.88 | ||
Completeness [%] | 99.9 | ||
Redundancy | 11.4 | ||
CC(1/2) | 0.999 | 1.000 | 0.179 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000MME, 0.1 M MES |