8V5S
VZV glycoprotein E C-terminal domain (cleaved) in complex with human Fab 5A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.077, 60.410, 114.667 |
| Unit cell angles | 90.00, 93.89, 90.00 |
Refinement procedure
| Resolution | 40.020 - 3.530 |
| R-factor | 0.2634 |
| Rwork | 0.261 |
| R-free | 0.30740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.020 | 3.660 |
| High resolution limit [Å] | 3.530 | 3.530 |
| Number of reflections | 16382 | 1136 |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 90.7 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.960 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.02 M Sodium/potassium phosphate 0.1 M Bis-Tris propane 7.5 20 % w/v PEG 3350 |
