8V52

Crystal structure of 2A10 Fab bound to Human TGF-beta3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.987
Spacegroup name	I 1 2 1
Unit cell lengths	133.940, 47.200, 200.830
Unit cell angles	90.00, 101.68, 90.00

Refinement procedure

Resolution	49.170 - 2.500
R-factor	0.223
Rwork	0.221
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.170	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	75332	3072
<I/σ(I)>	11
Completeness [%]	97.2
Redundancy	5.6
CC(1/2)	0.996	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	25% PEG 1000, 0.1M HEPES pH 7.5