8V4I
Structure of bovine anti-HIV Fab ElsE1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.789, 84.117, 174.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.890 - 1.810 |
R-factor | 0.2226 |
Rwork | 0.221 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.657 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.890 | 1.880 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.155 | 2.500 |
Rmeas | 0.162 | 2.640 |
Rpim | 0.046 | 0.772 |
Number of reflections | 45028 | 3292 |
<I/σ(I)> | 10.5 | 0.9 |
Completeness [%] | 95.6 | 70.1 |
Redundancy | 10.2 | 10 |
CC(1/2) | 0.996 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 40% ethylene glycol, 0.1M HEPES, pH 7.5, 5% PEG3000 |