8V3M
CCP5 apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-02-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 65.224, 80.925, 103.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.160 - 1.800 |
| R-factor | 0.1866 |
| Rwork | 0.186 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.930 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 1.658 |
| Rmeas | 0.091 | 1.801 |
| Rpim | 0.034 | |
| Number of reflections | 51328 | 2975 |
| <I/σ(I)> | 15.4 | 1.6 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 13.2 | 12.8 |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.15 M DL-Malic acid, pH7.0, 0.1 M imidazole, pH7.0, 16% PEG MME 550 |
