8V3J
Structure-Based Engineering of a Highly Immunogenic, Conformationally Stabilized FimH Antigen for a Urinary Tract Infection Vaccine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.040, 149.260, 99.350 |
| Unit cell angles | 90.00, 130.03, 90.00 |
Refinement procedure
| Resolution | 27.500 - 1.898 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (22-FEB-2023)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.086 | 2.168 |
| High resolution limit [Å] | 1.898 | 2.161 |
| Number of reflections | 86513 | 829 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 91.0 | 97.8 |
| Redundancy | 3.5 | 2.9 |
| CC(1/2) | 0.998 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M Sodium Acetate (pH 4.5), 25% (w/v) PEG 3350 |
