8V2X
Crystal Structure of the reconstruction of the worst case of the ancestral triosephosphate isomerase of the last opisthokont common ancestor obtained by bayesian inference
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-05 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.205, 59.535, 114.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.500 - 1.430 |
| R-factor | 0.1784 |
| Rwork | 0.178 |
| R-free | 0.17930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.039 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 58029 | 2619 |
| <I/σ(I)> | 23.9 | 2.7 |
| Completeness [%] | 98.0 | 90.6 |
| Redundancy | 5.4 | 3 |
| CC(1/2) | 0.997 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 3350 , 0.2 M NaF |
