8V1I

Crystal structure of human mascRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-29
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	137.063, 137.063, 135.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.990 - 2.300
R-factor	0.2154
Rwork	0.215
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.079
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.990	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	21925	2172
<I/σ(I)>	19.33
Completeness [%]	99.8
Redundancy	34
CC(1/2)	0.994	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M calcium chloride dihydrate, 0.1 M sodium acetate trihydrate and 20% (v/v) 2-propanol