8V11
Structure of a Saccharomyces cerevisiae Ipl1 peptide Bound to dwarf Ndc80 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2023-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0309 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 114.757, 114.757, 423.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.530 - 3.950 |
R-factor | 0.2696 |
Rwork | 0.267 |
R-free | 0.32660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.126 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.530 | 4.448 |
High resolution limit [Å] | 3.950 | 3.950 |
Rmerge | 0.556 | 3.899 |
Rmeas | 0.573 | 4.006 |
Rpim | 0.135 | 0.909 |
Number of reflections | 10981 | 785 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 42.6 | 10.5 |
Redundancy | 17.4 | 19 |
CC(1/2) | 0.993 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 15% polyethylene glycol 2000 monomethyl ether,500 mM sodium chloride,50 mM Tris pH 7.5 |