8V10

Structure of a Saccharomyces cerevisiae Mps1 peptide bound to dwarf Ndc80 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	77.36
Detector technology	PIXEL
Collection date	2023-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0309
Spacegroup name	C 2 2 21
Unit cell lengths	72.657, 168.295, 230.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	115.110 - 3.020
R-factor	0.2399
Rwork	0.238
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.349
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.110	3.398
High resolution limit [Å]	3.020	3.020
Rmerge	0.155	0.853
Rmeas	0.181	0.181
Rpim	0.068	0.637
Number of reflections	18187	909
<I/σ(I)>	5.3	1.6
Completeness [%]	65.0
Redundancy	6.1
CC(1/2)	0.994	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	291	13% polyethylene glycol 8000, 1M sodium chloride 100 mM PIPES pH 6.1