8UZT
Mitochondrial single-stranded binding protein bound to DNA
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 135.201, 135.201, 78.081 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.810 - 1.900 |
R-factor | 0.1855 |
Rwork | 0.185 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.847 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.810 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 55728 | 55728 |
<I/σ(I)> | 30 | |
Completeness [%] | 97.0 | |
Redundancy | 13.1 | |
CC(1/2) | 0.997 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350 and 200 mM sodium nitrate |