8UZP
Crystal Structure of the Computationally Designed Influenza Hemagglutinin Epitope Scaffold stem_mimetic_01 bound by Antibody CR9501
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 269.004, 55.943, 92.010 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 37.790 - 2.711 |
| R-factor | 0.1985 |
| Rwork | 0.199 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fqh 1ya9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.541 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.790 | 2.808 |
| High resolution limit [Å] | 2.711 | 2.711 |
| Rmerge | 0.140 | 0.678 |
| Rmeas | 0.168 | 0.794 |
| Rpim | 0.089 | 0.406 |
| Number of reflections | 36153 | 2434 |
| <I/σ(I)> | 13.38 | 2.9 |
| Completeness [%] | 90.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.814 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium citrate dibasic, 20% w/v PEG 3350 |
