8UVX
CosR DNA bound form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.125, 81.303, 68.073 |
| Unit cell angles | 90.00, 106.14, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.900 |
| R-factor | 0.2439 |
| Rwork | 0.241 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 13804 | 782 |
| <I/σ(I)> | 10.14 | |
| Completeness [%] | 87.7 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.980 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.02 M Sodium formate, 0.02 M Ammonium acetate, 0.02 M Sodiumcitrate, 0.02 M Sodiium potassium tartrate, 0.02 M Sodium oxamate, 0.01 M MES ph6.5, 40% v/v PEG 500 MME, 20% w/v PEG 20000 |
