8UVR
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.60A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.070, 447.770, 621.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 255.240 - 2.600 |
R-factor | 0.2206 |
Rwork | 0.218 |
R-free | 0.26450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.856 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 255.240 | 255.240 | 2.670 |
High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
Rmerge | 0.141 | 0.022 | 1.283 |
Rmeas | 0.161 | 0.025 | 1.462 |
Total number of observations | 7388156 | ||
Number of reflections | 1741371 | 20047 | 127943 |
<I/σ(I)> | 9.81 | 40.7 | 1.13 |
Completeness [%] | 98.8 | 96.4 | 98.5 |
Redundancy | 4.243 | 4.065 | 4.192 |
CC(1/2) | 0.995 | 0.999 | 0.247 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |