8UVR

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.070, 447.770, 621.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	255.240 - 2.600
R-factor	0.2206
Rwork	0.218
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.856
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	255.240	255.240	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.141	0.022	1.283
Rmeas	0.161	0.025	1.462
Total number of observations	7388156
Number of reflections	1741371	20047	127943
<I/σ(I)>	9.81	40.7	1.13
Completeness [%]	98.8	96.4	98.5
Redundancy	4.243	4.065	4.192
CC(1/2)	0.995	0.999	0.247

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME