8UUI

X-ray structure of Interleukin-23 in complex with peptide 23-446

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	110.740, 110.740, 85.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.480 - 2.430
R-factor	0.2254
Rwork	0.220
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX (dev_4788)
Refinement software	PHENIX (dev_4788)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.950	2.520
High resolution limit [Å]	2.430	2.430
Rmerge	0.081
Rmeas	0.030
Rpim	0.021
Number of reflections	44166	2379
<I/σ(I)>	19.8	1.2
Completeness [%]	100.0	100
Redundancy	15.3	14.2
CC(1/2)	1.000	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	293	19% PEG 1000, 0.1 M phosphate/citrate buffer pH 4.4, 0.2 M LiSO4