8UUD
BCX2627 complexed with human FVIIa and soluble Tissue Factor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-14 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.494, 82.870, 126.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.090 - 2.400 |
| R-factor | 0.20697 |
| Rwork | 0.204 |
| R-free | 0.26496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.583 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | CNX |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 2.400 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | |
| Number of reflections | 25452 | 1000 |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 90.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% (w/v)PEG 4K, 0.1M MgCl2, 0.1M ADA buffer, pH 6.5, VAPOR DIFFUSION, |
