8US8

Crystal structure of B1E11K malarial antibody in complex with RESA repeat peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033200
Spacegroup name	C 2 2 21
Unit cell lengths	78.704, 186.289, 131.517
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.670 - 2.560
R-factor	0.2173
Rwork	0.216
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.670	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	31520	3067
<I/σ(I)>	10.8
Completeness [%]	99.8
Redundancy	7.5
CC(1/2)	0.990	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M sodium acetate pH 5.2, 0.1 M NH4)2SO4, 25% (w/v) PEG4000