8URL

The Apo NanJ structure from Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-20
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	66.226, 48.804, 73.324
Unit cell angles	90.00, 93.95, 90.00

Refinement procedure

Resolution	22.890 - 2.200
R-factor	0.2032
Rwork	0.201
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (3.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	23654	1095
<I/σ(I)>	12.5
Completeness [%]	99.9
Redundancy	3.1
CC(1/2)	0.992	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M HEPES pH 7.5 2.0M (NH4)2SO4