8UQS

Crystal structure of the Opossum p53 tetramerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	38.275, 50.887, 60.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.310 - 1.350
R-factor	0.1716
Rwork	0.169
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.069
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.310	1.398
High resolution limit [Å]	1.350	1.350
Number of reflections	24100	1080
<I/σ(I)>	12.46
Completeness [%]	90.5
Redundancy	5.6
CC(1/2)	0.999	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1 M Tris pH 8.5, 20% PEG 1000