8UQQ

Structure of mCLIFY: a circularly permuted yellow fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 2 21 21
Unit cell lengths	35.020, 69.956, 84.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.340 - 1.570
R-factor	0.1794
Rwork	0.179
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.031
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.540	1.650
High resolution limit [Å]	1.570	1.570
Rmerge	0.153	1.741
Rmeas	0.160
Rpim	0.044	0.530
Number of reflections	26386	2232
<I/σ(I)>	9	1.6
Completeness [%]	89.3
Redundancy	12.7
CC(1/2)	0.998	0.121

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	296	0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% (w/v) PEG4000 and 18% (v/v) 2-Propanol