8UQ8

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 2-residue linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 31
Unit cell lengths	107.539, 107.539, 113.985
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.060 - 2.340
R-factor	0.1972
Rwork	0.196
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gb0 5egg
RMSD bond length	0.004
RMSD bond angle	0.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.340	2.340
Rmerge	0.109	0.849
Rmeas	0.117	0.849
Rpim	0.042	0.314
Number of reflections	61785	6078
<I/σ(I)>	21.25	4.42
Completeness [%]	99.5	98.54
Redundancy	7.6	7.2
CC(1/2)	0.998	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	Crystals were obtained by mixing 2 microliters of the protein fusion (9.5 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 0.1 M HEPES, pH 7.5, 2.4 M NaCl