8UPI

Structure of a periplasmic peptide binding protein from Mesorhizobium sp. AP09 bound to aminoserine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	Agilent SuperNova
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-06
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 21 21 21
Unit cell lengths	56.247, 58.920, 139.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.510 - 1.550
R-factor	0.1815
Rwork	0.179
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.801
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.510	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.078	0.503
Rmeas	0.089	0.634
Rpim	0.041	0.378
Total number of observations	254272	9261
Number of reflections	67507	3194
<I/σ(I)>	7.8	2.1
Completeness [%]	99.2
Redundancy	3.8	2.9
CC(1/2)	0.985	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% PEG 4000