8UP4

Crystal structure of Lsd18 (a flavin-dependent monooxygenase)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-12
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000000
Spacegroup name	P 1
Unit cell lengths	46.354, 61.790, 75.355
Unit cell angles	74.64, 81.70, 77.11

Refinement procedure

Resolution	36.749 - 1.510
R-factor	0.142
Rwork	0.140
R-free	0.17850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.450	1.590
High resolution limit [Å]	1.510	1.510
Rmerge	0.058	0.416
Rmeas	0.069	0.496
Rpim	0.036	0.266
Number of reflections	117632	16922
<I/σ(I)>	11.6	2.9
Completeness [%]	95.9	94.1
Redundancy	3.5	3.4
CC(1/2)	0.998	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Tris pH8.5, 0.85 M NaCl, 32% (w/v) PEG4000