8UP2

Murine Fab JAR 4 bound to meningococcal Factor H binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 1 21 1
Unit cell lengths	74.430, 67.440, 168.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.430 - 1.600
R-factor	0.1877
Rwork	0.187
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.102
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.430	1.660
High resolution limit [Å]	1.600	1.603
Rmerge	0.164	1.366
Rpim	0.070	0.587
Number of reflections	218509	133437
<I/σ(I)>	5.84	1.32
Completeness [%]	99.9	99.53
Redundancy	9.4	6.1
CC(1/2)	0.991	0.489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.05M NaOAc 20% PEG 4,000 0.085M Tris Cl, pH 8.5 15% glycerol