8UO6

HIV-1 Rev Response Element (RRE) Stem-Loop II (SLII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	67.782, 81.314, 81.372
Unit cell angles	90.00, 99.14, 90.00

Refinement procedure

Resolution	57.150 - 2.850
R-factor	0.2326
Rwork	0.231
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.756
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.200	3.200
High resolution limit [Å]	2.850	2.850
Rmeas	0.100
Rpim	0.050	0.720
Number of reflections	12674	635
<I/σ(I)>	8.9	1
Completeness [%]	88.5
Redundancy	4
CC(1/2)	0.999	0.487

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	100 mM SPG (mixture of succinic acid, sodium dihydrogen phosphate, and glycine in a 2:7:7 molar ratio), 20-24% polyethylene glycol-6000, and 200 mM sodium fluoride