8UKT

RNA polymerase II elongation complex with Fapy-dG lesion with AMP added

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-25
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9774
Spacegroup name	C 1 2 1
Unit cell lengths	156.681, 221.393, 190.539
Unit cell angles	90.00, 97.67, 90.00

Refinement procedure

Resolution	47.750 - 3.600
R-factor	0.2522
Rwork	0.251
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.686
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.750	3.650
High resolution limit [Å]	3.580	3.580
Rmerge	0.581	3.668
Rmeas	0.627	3.974
Rpim	0.236	1.519
Total number of observations	528303	30534
Number of reflections	75622	4498
<I/σ(I)>	4.8	0.6
Completeness [%]	100.0
Redundancy	7	6.8
CC(1/2)	0.966	0.218

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000