8UKH
Crystal structure of Plasmodium falciparum CelTOS in complex with antibody 4h12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.689, 59.728, 153.445 |
| Unit cell angles | 82.07, 82.20, 72.27 |
Refinement procedure
| Resolution | 19.860 - 3.520 |
| R-factor | 0.269 |
| Rwork | 0.268 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.649 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.520 |
| Number of reflections | 23861 | 2292 |
| <I/σ(I)> | 6.28 | |
| Completeness [%] | 97.0 | |
| Redundancy | 1.98 | |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2 M lithium acetate and 21% PEG 3350 |
