8UK7
Periplasmic domain of Escherichia coli CpxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03316 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.160, 43.163, 186.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.190 - 1.800 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 1.090 |
| Rmeas | 0.076 | 1.160 |
| Rpim | 0.028 | 0.408 |
| Number of reflections | 28337 | 2769 |
| <I/σ(I)> | 13.75 | 2.68 |
| Completeness [%] | 98.1 | 96.6 |
| Redundancy | 8 | 7.9 |
| CC(1/2) | 0.997 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | 100 mM Tris-HCl pH 7.5, 350 mM NaCl, 20% v/v 2-methyl-2,4-pentanediol |
