8UGT

E. eligens beta-glucuronidase bound to UNC10206581-G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 64 2 2
Unit cell lengths	179.938, 179.938, 132.792
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.980 - 2.650
R-factor	0.2401
Rwork	0.239
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.252
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.980	2.745
High resolution limit [Å]	2.650	2.650
Rmerge	0.035	0.513
Rmeas	0.050	0.725
Rpim	0.035	0.513
Number of reflections	37256	3623
<I/σ(I)>	13.74	1.36
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.999	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.075 M HEPES, pH 7.5, 15 % (w/v) PEG 1000