8UGT
E. eligens beta-glucuronidase bound to UNC10206581-G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 179.938, 179.938, 132.792 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.980 - 2.650 |
R-factor | 0.2401 |
Rwork | 0.239 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.252 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.980 | 2.745 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.035 | 0.513 |
Rmeas | 0.050 | 0.725 |
Rpim | 0.035 | 0.513 |
Number of reflections | 37256 | 3623 |
<I/σ(I)> | 13.74 | 1.36 |
Completeness [%] | 100.0 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.075 M HEPES, pH 7.5, 15 % (w/v) PEG 1000 |