8UDZ
The Structure of LTBP-49247 Fab Bound to TGFbeta1 Small Latent Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2021-10-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 73.495, 141.905, 186.122 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.500 - 2.210 |
R-factor | 0.2333 |
Rwork | 0.232 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | xia2 |
Data scaling software | XDS |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.500 | 2.289 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.099 | 3.364 |
Rmeas | 0.103 | 3.502 |
Rpim | 0.029 | 0.967 |
Number of reflections | 98200 | 9725 |
<I/σ(I)> | 13.3 | 0.4 |
Completeness [%] | 99.5 | 94.77 |
Redundancy | 13.1 | 12.9 |
CC(1/2) | 0.997 | 0.351 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 15% PEG 3350, 12% Tacsimate (pH 4.0), 10 mM Magnesium Chloride |