8UDZ

The Structure of LTBP-49247 Fab Bound to TGFbeta1 Small Latent Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-10-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 2 21 21
Unit cell lengths	73.495, 141.905, 186.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.500 - 2.210
R-factor	0.2333
Rwork	0.232
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.500	2.289
High resolution limit [Å]	2.210	2.210
Rmerge	0.099	3.364
Rmeas	0.103	3.502
Rpim	0.029	0.967
Number of reflections	98200	9725
<I/σ(I)>	13.3	0.4
Completeness [%]	99.5	94.77
Redundancy	13.1	12.9
CC(1/2)	0.997	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	15% PEG 3350, 12% Tacsimate (pH 4.0), 10 mM Magnesium Chloride