8UD8

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.010, 449.040, 621.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.890 - 2.600
R-factor	0.2182
Rwork	0.216
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8g29
RMSD bond length	0.004
RMSD bond angle	0.827
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	121.890	121.890	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.166	0.046	1.739
Rmeas	0.184	0.051	1.925
Total number of observations	9142845
Number of reflections	1722763	19181	128160
<I/σ(I)>	8.19	28.88	0.96
Completeness [%]	97.0	91.5	97.9
Redundancy	5.307	5.61	5.41
CC(1/2)	0.993	0.998	0.156

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME