8UD0

Sterile Alpha Motif (SAM) domain from Tric1 from Arabidopsis thaliana - G241E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953732
Spacegroup name	P 21 21 21
Unit cell lengths	46.579, 58.537, 61.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.550 - 1.890
R-factor	0.2017
Rwork	0.200
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.550	1.960
High resolution limit [Å]	1.890	1.890
Rmerge	0.090	1.500
Rpim	0.030	0.420
Number of reflections	14037	1355
<I/σ(I)>	18.9	2.41
Completeness [%]	99.6	96.1
Redundancy	13.1	13
CC(1/2)	0.999	0.077

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	293.15	1.8-2.3 M ammonium sulphate, 0.1 M Tris