8U7F

Crystal structure of CIB_12 beta-galactosidase from Cuniculiplasma divulgatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-10-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	193.215, 51.805, 128.794
Unit cell angles	90.00, 108.34, 90.00

Refinement procedure

Resolution	24.930 - 2.550
R-factor	0.2217
Rwork	0.220
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.093	0.581
Rpim	0.047	0.289
Number of reflections	38156	1629
<I/σ(I)>	18.34	1.54
Completeness [%]	95.2	81.5
Redundancy	4.9	4.7
CC(1/2)		0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	PEG 3350 25%, 0.2 M sodium tartrate, 0.1 M Tris pH 8.5, 0.3 M NDSB 201, 1% 1-butyl-2,3-dimethylimidazolium tetrafluoroborate