8U21
A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP E6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97741 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.647, 146.083, 50.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.580 - 1.960 |
| R-factor | 0.1793 |
| Rwork | 0.177 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.078 | 0.042 | 0.560 |
| Total number of observations | 92961 | ||
| Number of reflections | 23695 | 1287 | 1172 |
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.7 | 99.2 | 100 |
| Redundancy | 3.9 | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.25M (NH4)2SO4, 0.1M Sodium acetate pH 5.0, 30% PEG4K, 5% Glycerol |
