8U1F

FGFR2 Kinase Domain Bound to Irreversible Inhibitor Cmpd 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	62.513, 88.280, 129.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.110 - 3.330
R-factor	0.2689
Rwork	0.268
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.709
Data reduction software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.110	3.140
High resolution limit [Å]	3.060	3.060
Rmerge	0.136
Number of reflections	14247	6280
<I/σ(I)>	4.5
Completeness [%]	97.1
Redundancy	5.5
CC(1/2)	0.970	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	0.1 M Tris pH 8, 18% PEG K