8U17

The ternary complex structure of DDB1-CRBN-SALL4(ZF1,2)-long bound to Pomalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	116.077, 151.660, 195.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.900 - 3.100
R-factor	0.3069
Rwork	0.305
R-free	0.34560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.510
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.063	9.900
Rmeas	0.282	12.093
Rpim	0.080	3.431
Total number of observations	788438
Number of reflections	63326	4418
<I/σ(I)>	7.4
Completeness [%]	100.0
Redundancy	12.5	12.3
CC(1/2)	0.996	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Sodium malonate, 20% PEG 3350