8U16
The ternary complex structure of DDB1-CRBN-SALL4(ZF1,2)-short bound to Pomalidomide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.536, 151.986, 218.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.350 - 2.900 |
R-factor | 0.2217 |
Rwork | 0.219 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.700 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.077 | 8.198 |
Rmeas | 0.609 | 8.365 |
Rpim | 0.118 | 1.660 |
Total number of observations | 1886081 | 113305 |
Number of reflections | 71316 | 4497 |
<I/σ(I)> | 5 | 0.6 |
Completeness [%] | 100.0 | |
Redundancy | 26.4 | 25.2 |
CC(1/2) | 0.995 | 0.432 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Sodium malonate, 20% PEG 3350 |