8U0P

Synaptic complex of human DNA polymerase Lambda DL variant engaged on a noncomplementary DNA double-strand break

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.205, 60.068, 140.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.400 - 1.900
R-factor	0.1734
Rwork	0.172
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.719
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmeas	0.090	0.812
Rpim	0.037	0.347
Number of reflections	38985	1913
<I/σ(I)>	19.29	1.58
Completeness [%]	99.5	99.7
Redundancy	5.7	5.2
CC(1/2)	0.996	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	85 mM Na cacodylate pH 6.5, 0.17 M ammonium sulfate, 25.5 % (w/v) PEG 8000, 15% (v/v) glycerol