8U0F
Bacterial fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | H 3 |
| Unit cell lengths | 186.610, 186.610, 59.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.590 - 2.070 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.326 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.770 | 2.180 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rpim | 0.129 | 0.685 |
| Number of reflections | 46942 | 6827 |
| <I/σ(I)> | 6.9 | 2.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.9 | 3.6 |
| CC(1/2) | 0.964 | 0.209 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate, pH 4.5, 1.2 M ammonium sulfate, 4% w/v benzamidine hydrochloride |
