8TYZ
Structure of a bacterial E1-E2-Ubl complex (form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 199.925, 199.925, 66.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.680 - 2.680 |
R-factor | 0.2093 |
Rwork | 0.207 |
R-free | 0.24460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.007 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 199.930 | 2.800 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.101 | 3.776 |
Number of reflections | 38727 | 4641 |
<I/σ(I)> | 19.2 | 0.7 |
Completeness [%] | 100.0 | |
Redundancy | 13.7 | |
CC(1/2) | 1.000 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM imidazole pH 8.0, 200 mM MgCl2, and 10% PEG 3350 |